T CELL MEDIATED
CYTOTOXICITY
Our assays will advance the development of your therapeutic candidates
Want to talk to one of our team about T Cell Mediated Cytotoxicity?
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- In consultation with you, we design our assays to provide the fullest information on your molecule
- We have developed a proprietary range of target cell lines, have access to a range of pre-screened primary T cell donors and can offer a choice of readouts – kinetic or endpoint analysis
- Our capabilities allow us to support early screening activities all the way through to the development of lot release potency assay methods
Our T cell mediated cytotoxicity assays can expedite your candidate selection process
Find out how we can support your T Cell engager
can be measured in many different ways each with their own benefits
What are the Target Cell Considerations?
A number of options are available for the source of target cells for our T cell mediated cytotoxicity assays.
We have a variety of target cells at our disposal
Engineered cell lines may be the preferred option.
An immortalised cell line expressing your target of interest may not be available or may require complicated induction protocols
For a simpler, more sensitive assay system, immortalised cell lines are available and represent a fundamental model for characterisation of T cell mediated cytotoxicity
We also provide the option of primary tumour tissue.
This allows the development of a disease relevant system, potentially more predictive of in vivo efficacy
Our in house cell line development capabilities allow us to develop bespoke cell lines to meet your requirements.
We have a library of target antigens
Find out how we can support your T Cell engager
Effector Cell Considerations
The selection of an appropriate effector cell population is critical to the design of
T cell mediated cytotoxicity assays
Factors for consideration include:
The cell source i.e. PBMCs – fresh or cryopreserved?
Isolated effector cell population – pan-T cells, CD8+ or CD3+ enirched?
Should the effectors be activated or pre-expanded?
How will the disease status of the donor affect the assay?
We are experienced in performing assays with a range of effector cell populations and have access to a variety of pre-screened donors.
We can advise you on the best choice of effector cell population for your requirements
What are the assay readout considerations?
Real-Time Cell Analysis
Real-time, kinetic measurement of T cell mediated target cell death, offering a wealth of information in a label-free manner. Fast becoming the preferred method for measuring cytotoxicity
CYTOKINE
RELEASE
T REG
SUPPRESSION
MIXED LYMPHOCYTE
REACTION (MLR)
T CELL MEDIATED
CYTOTOXICITY
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