Test your candidates in a more physiologically relevant in vitro system.

Exhausted T Cell Assay

Antibody Analytics have developed a model assay system which mimics the T cell status observed in cancer settings. Using our exhausted T cell model, we can help you determine how well your therapeutic candidates will perform in a more challenging system, where the T cells are phenotypically and functionally akin to those found in the tumor micro-environment.

Want to learn more about our Exhausted T Cell model?
Contact one of our team now to see how we can help

We’re always looking for passionate, talented & driven people. Regardless of whether your happy place is in the Labs, Operations, Quality or Marketing. Head on over to the Careers Page to see our latest openings.

What is T cell Exhaustion?

T cell exhaustion is a phenotypic and functional state of a T cell. It is characterised by increased cell surface expression of checkpoint inhibitors and a reduced functional capacity.

Exhausted T cells exhibit reduced proliferation, a reduction in cytokine production and function. In the presence of repeated antigenic stimulation in the context of chronic infection or cancer, increasing numbers of T cells reach this exhausted state and are less able to perform their intended anti-viral or anti-tumor functions.

How does our Exhausted T cell model work?

1. T cells are isolated from PBMCs and are stimulated in culture via CD3 and CD28.

2. Repeated stimulations produce T cells which exhibit sustained up-regulation of multiple checkpoint inhibitors PD-1, TIM-3, LAG-3, CTLA-4, BTLA and the transcription factor TOX

3. These cells are not anergic but are comparatively less proliferative, cytotoxic and able to produce IFNγ and IL-2 than resting T cells

4. In the presence of your therapeutic candidate, the exhausted T cells can be plugged into a functional assay of your choice; T cell cytotoxicity, T cell activation or mixed lymphocyte reactions

Exhausted T Cell Assay

How are customer’s using the T cell exhaustion assay?

Although a key target for immune checkpoint blockade, exhausted T cell phenotypes are not well comprehended from a therapeutic point of view. 

Immuno-oncology-based developers are looking to understand the pathways and molecular signatures governing the T cell exhaustion, in an effort to restore anti-tumour immunity. 

Customers use our exhausted T cell exhaustion platforms to determine whether their therapeutic candidates can rescue or revitalize the functionality of the T cells in our human in vitro model, or when they are assessing their candidate’s ability to halt the progression of exhaustion, whereby their molecules are present during the in vitro exhaustion process. 

In both approaches the T cells are phenotypically assessed by flow cytometry for expression of exhaustion markers, activation markers and any others deemed relevant to the mode of action of the customer’s molecules. Changes in cytokine production, proliferative response and functionality are all assessed to give the customer a clear understanding of how their molecule interacts and functions within a compromised tumour microenvironment model albeit an in vitro one. 

Working with us

Although every project is different, here at Antibody Analytics we have built a systematic approach to delivering complex customer studies while keeping the customer’s research needs at the heart of our assay set ups. Our T cell exhaustion methods have been standardized to allow our customers to insert their molecules into a method which is able to answer their developability and functional questions routinely and consistently. Alternatively, we can create a bespoke set up which is unique to you and the challenges you face. 

If you are looking to test your molecule in a more predictable, standardized method, why not share some of your requirements in the form below and we can get a proposal out to you, or alternatively speak to our team and let us build a tailored approach to just for you.  

Our Blog

Meet Jennifer Lawson, Chief Operating Officer 

Meet Jennifer Lawson, Chief Operating Officer 

“How did it all begin? I studied for an undergraduate degree in Immunology at the University of Glasgow. When I graduated in 2001, I did what many aspiring scientists do; I put on my lab coat to get some hands on experience in a proper lab.  “I took my knowledge...

read more
World Day for Safety and Health at Work 2022 

World Day for Safety and Health at Work 2022 

As scientists, we have an innate desire and duty of care to protect and prevent accidents and diseases. In doing so, we have developed a strong occupational safety and health (OSH) culture that values and safeguards our employees. Built on inclusion, we work together to find effective, appropriate, and sustainable solutions that promote the right to health and safety in the laboratory. 

read more
The Second to None Secondment

The Second to None Secondment

As agents of change, we’ve switched things up in the laboratory. To celebrate this period of change, we’ve sat down with Hannah Findlay to see how her secondment into the Research and Development (R&D) department aligns with the continual growth of Antibody Analytics.

read more
From Operations to R&D in 400 days

From Operations to R&D in 400 days

As you’ve probably heard, Antibody Analytics is expanding, and so is our development opportunities. To celebrate our growth, we’ve sat down with a very talented lady who, with her hard work and determination, has attained a secondment in the Research and Development...

read more